LD 50 Lab: How does the effect of the concentration of toxin effect the survival rate of Artemiidae artemia
Students will complete a laboratory that investigate how the concentration of a toxin can effect the survival rate of Artemiidae artemia, the common brine shrimp.
Objectives
Students will investigate how toxins can effect the life span of an organim
Materials
5 beakers pH paper eyedropper live brine shrimp solutions in a range of pH magnifying glass goggles
Procedure
1. measure the pH of each of the solutions
2. observe the activity of the shrimp in their water (control)
3. add a drop of water with brine shrimp to each solution
4. observe the activity of the shrimp in each solution
5. record results
References
State Standards
1.3.2- The student will recognize safe laboratory procedures.
1.3.3- The student will demonstrate safe handling of the chemicals and materials of science.(NTB)
1.4.1- The student will organize data appropriately using techniques such as tables, graphs, and webs (for graphs: axes labeled with appropriate quantities, appropriate units on axes, axes labeled with appropriate intervals, independent and dependent variables on correct axes, appropriate title).
1.4.2- The student will analyze data to make predictions, decisions, or draw conclusions.
1.4.6- The student will describe trends revealed by data.
3.1.2- The student will be able to discuss factors involved in the regulation of chemical activity as part of a homeostatic mechanism.
3.5.1- The student will analyze the relationships between biotic diversity and abiotic factors in environments and the resulting influence on ecosystems.
3.5.2- The student will analyze the interrelationships and interdependencies among different organisms and explain how these relationships contribute to the stabilty of the ecosystem.
4.1.2- The student will gather and interpret data related to physical and chemical properties of matter such as density and percent composition. AT LEAST: constructing data tables, graphing linear relationship, appropriate technology to analyze data.
Lesson Resources